Discrimination of boar seminal plasma protein precipitin factor using plant precipitin

Proteomics of boar seminal plasma

Equilibria in Precipitin Reactions

1. In these studies several phases of the predpitm reactions were investigated by the use of purified proteins as antigens. These preparations were edestin from hemp-seed and crystalline ovalbumin from fresh eggs. The ovalbumin, isolated by the method of Hopkins and Pinkus,.was apparently as pure as is obtainable by chemical means. This albumin, however, produced moderately severe anaphylactic ...

Agglutination by Precipitin

Serum (antigen) when heated at a temperature sufficient to cause definite clouding reacts more intensely with a specific precipitin than a portion of the unheated serum or samples heated at lower temperatures. The phenomenon is explained on the basis that coagulated protein in suspension is covered with undenatured antigen and the addition of precipitin causes agglutination of the coagulated pr...

Studies on the Precipitin Reaction

1. Partial hydrolysis products of the specific polysaccharide of Type III pneumococcus ranging from 550 to 1,800 in formula weight can be quantitatively freed from unhydrolyzed polysaccharide. 2. The fractions yield specific precipitates with Type III antipneumococcus horse serum but fail to precipitate homologous rabbit antisera, giving rise only to specific inhibition. The aldobionic acid, th...

Quantitative aspects of the M protein capillary precipitin test.

A capillary procedure for quantitatively determining M protein is described. Capillaries were filled with measured amounts of serum and streptococcal extract. The capillaries were incubated, and then centrifuged to pack the precipitates. The relative sizes of the precipates were compared by a determination of the weights of their paper images (obtained by reflection from a microscope). Meaningf...